Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
27640-1-AP targets PIP5K1C in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26525 Product name: Recombinant human PIP5K1C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 480-573 aa of NM_001195733 Sequence: MIPSEREEAQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSETSEQPRYRRRTQSSGQDGRPQEEPPAEEDLQ 相同性解析による交差性が予測される生物種 |
| Full Name | phosphatidylinositol-4-phosphate 5-kinase, type I, gamma |
| Calculated molecular weight | 73 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | NM_001195733 |
| Gene Symbol | PIP5K1C |
| Gene ID (NCBI) | 23396 |
| RRID | AB_2880936 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60331 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PIP5K1C antibody 27640-1-AP | Download protocol |
| WB protocol for PIP5K1C antibody 27640-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer. | ||
Cell Signal The miR-424-3p/PIP5K1C axis regulates AKT phosphorylation and granulosa cell proliferation. | ||
J Immunol Res PIPKIγ Regulates CCL2 Expression in Colorectal Cancer by Activating AKT-STAT3 Signaling. |