Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15713-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15713-1-AP
WB result of PIP5K1A antibody (15713-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIP5K1A transfected HepG2 cells.
WB analysis of HeLa using 15713-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15713-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15713-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa cells using 15713-1-AP
IP result of anti-PIP5K1A (15713-1-AP for IP and Detection).
IP experiment of mouse heart using 15713-1-AP
IP result of anti-PIP5K1A (IP:15713-1-AP, 4ug; Detection:15713-1-AP 1:500) with mouse heart tissue lysate 3200ug.
IHC staining of human tonsillitis using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP (PIP5K1A antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP (PIP5K1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP (PIP5K1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 15713-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15713-1-AP
Immunofluorescent analysis of Hela cells, using PIP5K1A antibody 15713-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

PIP5K1A Polyclonal antibody

PIP5K1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15713-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha, EC:2.7.1.68, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, PIP5K1 alpha

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, human heart tissue, human liver tissue, NCI-H1299 cells, PC-12 cells
Positive IP detected in	HeLa cells, mouse heart tissue
Positive IHC detected in	human tonsillitis tissue, human placenta tissue, mouse pancreas tissue, rat heart tissue, mouse kidney tissue, mouse heart tissue, human ovary cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 11 publications below
IHC	See 2 publications below
IF	See 6 publications below
IP	See 2 publications below

Product Information

15713-1-AP targets PIP5K1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8340 Product name: Recombinant human PIP5K1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-500 aa of BC007833 Sequence: SLCSEPLIELCSSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH 相同性解析による交差性が予測される生物種
Full Name	phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Calculated molecular weight	56 kDa, 63 kDa
Observed molecular weight	63 kDa
GenBank accession number	BC007833
Gene Symbol	PIP5K1A
Gene ID (NCBI)	8394
RRID	AB_2164696
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99755
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Protocols

Product Specific Protocols
IF protocol for PIP5K1A antibody 15713-1-AP	Download protocol
IHC protocol for PIP5K1A antibody 15713-1-AP	Download protocol
IP protocol for PIP5K1A antibody 15713-1-AP	Download protocol
WB protocol for PIP5K1A antibody 15713-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB,IF	Nat Cell Biol Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation. Authors - Rong Yueguang Y View Article
human	WB,IP,IF	Cancer Res β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages Authors - Daqi Li KD Validated View Article
		Autophagy IL17A disrupts autophagy-lysosomal function and lysosome reformation through the GSK3B-TFE3 signaling pathway in Huntington disease. Authors - Kai-Po Chen View Article
human	WB	J Clin Invest Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation. Authors - Jaerak Chang View Article
human	IF	Adv Sci (Weinh) Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Authors - Antoni Gañez‐zapater View Article
mouse,human	WB,IF	Cell Death Dis Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling Authors - Eric Toh View Article

Validation Data Gallery

WB analysis using 15713-1-AP

WB analysis of HepG2 using 15713-1-AP

WB analysis of HeLa using 15713-1-AP

WB analysis of human liver using 15713-1-AP

WB analysis of human heart using 15713-1-AP

IP experiment of HeLa cells using 15713-1-AP

IP experiment of mouse heart using 15713-1-AP

IHC staining of human tonsillitis using 15713-1-AP

IHC staining of human tonsillitis using 15713-1-AP

IHC staining of human tonsillitis using 15713-1-AP

IHC staining of mouse heart using 15713-1-AP

IHC staining of human ovary cancer using 15713-1-AP

IHC staining of human placenta using 15713-1-AP

IHC staining of mouse kidney using 15713-1-AP

IHC staining of mouse pancreas using 15713-1-AP

IHC staining of rat heart using 15713-1-AP

IF Staining of HeLa using 15713-1-AP

PIP5K1A Polyclonal antibody

PIP5K1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation.

β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages

IL17A disrupts autophagy-lysosomal function and lysosome reformation through the GSK3B-TFE3 signaling pathway in Huntington disease.

Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.

Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation.

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling

Able™

Able^™