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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIP4K2B Recombinant antibody (83647-3-RR)

    WB analysis using 83647-3-RR (same clone as 83647-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83647-3-RR (PIP4K2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PIP4K2B Recombinant antibody (83647-3-RR)

    IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PIP4K2B Recombinant antibody (83647-3-RR)

    IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PIP4K2B Recombinant antibody (83647-3-RR)

    IF Staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PIP4K2B antibody (83647-3-RR, Clone: 240645F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using PIP4K2B Recombinant antibody (83647-3-RR)

    FC experiment of A549 using 83647-3-RR (same clone as 83647-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PIP4K2B Recombinant antibody (83647-3-RR, Clone:240645F10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

PIP4K2B Recombinant monoclonal antibody, PBS Only

PIP4K2B Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 83647-3-PBS
Clone No.240645F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Synonyms

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83647-3-PBS targets PIP4K2B in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4099

Product name: Recombinant human PIP4K2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-281 aa of BC027459

Sequence: MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEEILVLSPGRRIA

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol-5-phosphate 4-kinase, type II, beta
Calculated molecular weight 416 aa, 47 kDa
Observed molecular weight45 kDa
GenBank accession numberBC027459
Gene Symbol PIP4K2B
Gene ID (NCBI) 8396
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP78356
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer.

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