Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, A431 cells, C6 cells, NIH/3T3 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 8 publications below |
Product Information
12368-1-AP targets PINX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PINX1 fusion protein Ag3043 相同性解析による交差性が予測される生物種 |
| Full Name | PIN2-interacting protein 1 |
| Calculated molecular weight | 328 aa, 37 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC015479 |
| Gene Symbol | PINX1 |
| Gene ID (NCBI) | 54984 |
| RRID | AB_2164405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BK5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PINX1, also named as LPTL, LPTS and Protein 67-11-3, is a Liver-related putative tumor suppressor. It belongs to the PINX1 family. PINX1 is a microtubule-binding protein essential for faithful chromosome segregation. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. PINX1 inhibits telomerase activity. It may inhibit cell proliferation and act as tumor suppressor. PINX1 has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, but the expression status in epithelial ovarian tumors has not been investigated.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PINX1 antibody 12368-1-AP | Download protocol |
| IF protocol for PINX1 antibody 12368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation.
| ||
Mol Cancer The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway. | ||
Am J Cancer Res PINX1 promotes malignant transformation of thyroid cancer through the activation of the AKT/MAPK/β-catenin signaling pathway.
| ||
Mol Cancer PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway. | ||
Cancer Sci Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma. |