Validation Data Gallery

Filter: AllWBIHC

WB analysis of rat lymph using 13329-1-AP
rat lymph tissue were subjected to SDS PAGE followed by western blot with 13329-1-AP (PI3K p101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 13329-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13329-1-AP (PI3K p101 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 13329-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13329-1-AP (PI3K p101 antibody at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 13329-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13329-1-AP (PI3K p101 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 13329-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13329-1-AP (PI3K p101 antibody at dilution of 1:50 (under 40x lens).

PI3K p101 Polyclonal antibody

PI3K p101 Polyclonal Antibody for ELISA

Cat No. 13329-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

F730038I15Rik, FOAP 2, p101, P101 PI3K, PI3 kinase p101 subunit, PI3K p101, PIK3R5, Protein FOAP 2, PtdIns 3 kinase p101

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 16 publications below

Product Information

13329-1-AP targets PI3K p101 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4137 Product name: Recombinant human PIK3R5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 532-880 aa of BC028212 Sequence: RAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP 相同性解析による交差性が予測される生物種
Full Name	phosphoinositide-3-kinase, regulatory subunit 5
Calculated molecular weight	880 aa, 97 kDa
GenBank accession number	BC028212
Gene Symbol	PIK3R5/PI3K p101
Gene ID (NCBI)	23533
RRID	AB_10638433
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WYR1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for PI3K p101 antibody 13329-1-AP	Download protocol
WB protocol for PI3K p101 antibody 13329-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,rabbit	WB	Environ Res Effects of external environment on promoter methylation of PIK3R5 and related pathway regulation in steroid-induced femoral head necrosis Authors - Wentao Qin View Article
mouse	WB	Allergy miRNA-155 controls mast cell activation by regulating the PI3Kγ pathway and anaphylaxis in a mouse model. Authors - K Biethahn View Article
mouse	WB	J Ethnopharmacol Dissecting the mechanisms of velvet antler extract against diabetic osteoporosis via network pharmacology and proteomics Authors - Mingyue Wang View Article
human	WB	Oncol Rep Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways. Authors - Huang Li View Article
mouse	WB	Cell Tissue Res Lycium barbarum polysaccharide attenuates diabetic testicular dysfunction via inhibition of the PI3K/Akt pathway-mediated abnormal autophagy in male mice. Authors - Guang-Jiang Shi View Article
human	WB	Chin Med Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo. Authors - Hong-Yan Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of rat lymph using 13329-1-AP

IHC staining of human lung cancer using 13329-1-AP

IHC staining of human lung cancer using 13329-1-AP

IHC staining of human liver cancer using 13329-1-AP

IHC staining of human liver cancer using 13329-1-AP

PI3K p101 Polyclonal antibody

PI3K p101 Polyclonal Antibody for ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Effects of external environment on promoter methylation of PIK3R5 and related pathway regulation in steroid-induced femoral head necrosis

miRNA-155 controls mast cell activation by regulating the PI3Kγ pathway and anaphylaxis in a mouse model.

Dissecting the mechanisms of velvet antler extract against diabetic osteoporosis via network pharmacology and proteomics

Hedyotis diffusa Willd. inhibits VEGF‑C‑mediated lymphangiogenesis in colorectal cancer via multiple signaling pathways.

Lycium barbarum polysaccharide attenuates diabetic testicular dysfunction via inhibition of the PI3K/Akt pathway-mediated abnormal autophagy in male mice.

Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.