Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, Jurkat cells |
| Positive IF/ICC detected in | LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IP | See 1 publications below |
Product Information
67964-1-Ig targets PI3 Kinase p110 Delta in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26995 Product name: Recombinant human PIK3CD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 395-580 aa of BC132919 Sequence: YAVIEKAKKARSTKKKSKKADCPIAWANLMLFDYKDQLKTGERCLYMWPSVPDEKGELLNPTGTVRSNPNTDSAAALLICLPEVAPHPVYYPALEKILELGRHSECVHVTEEEQLQLREILERRGSGELYEHEKDLVWKLRHEVQEHFPEALARLLLVTKWNKHEDVAQMLYLLCSWPELPVLSAL 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoinositide-3-kinase, catalytic, delta polypeptide |
| Calculated molecular weight | 1044 aa, 120 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC132919 |
| Gene Symbol | PIK3CD |
| Gene ID (NCBI) | 5293 |
| RRID | AB_2918715 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O00329 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PI3 Kinase p110 Delta antibody 67964-1-Ig | Download protocol |
| WB protocol for PI3 Kinase p110 Delta antibody 67964-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |