×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of LNCaP cells using PI3 Kinase p110 Delta Monoclonal antibody (67964-1-Ig)

    WB analysis of LNCaP using 67964-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PI3 Kinase p110 Delta Monoclonal antibody (67964-1-Ig)

    WB analysis of Jurkat using 67964-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using PI3 Kinase p110 Delta Monoclonal antibody (67964-1-Ig)

    IF Staining of LNCaP using 67964-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using PIK3CD antibody (67964-1-Ig, Clone: 2G11C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PI3 Kinase p110 Delta Monoclonal antibody

PI3 Kinase p110 Delta Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67964-1-Ig
Clone No.2G11C6
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells
Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67964-1-Ig targets PI3 Kinase p110 Delta in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26995

Product name: Recombinant human PIK3CD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 395-580 aa of BC132919

Sequence: YAVIEKAKKARSTKKKSKKADCPIAWANLMLFDYKDQLKTGERCLYMWPSVPDEKGELLNPTGTVRSNPNTDSAAALLICLPEVAPHPVYYPALEKILELGRHSECVHVTEEEQLQLREILERRGSGELYEHEKDLVWKLRHEVQEHFPEALARLLLVTKWNKHEDVAQMLYLLCSWPELPVLSAL

相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, delta polypeptide
Calculated molecular weight 1044 aa, 120 kDa
Observed molecular weight110 kDa
GenBank accession numberBC132919
Gene Symbol PIK3CD
Gene ID (NCBI) 5293
RRIDAB_2918715
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO00329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
WB protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Med Genomics

Identification of serum miR‑320a in gestational diabetes mellitus for predicting macrosomia and in vitro mechanistic investigation.

Authors - Ruiping Yu
View Article
mouseIP

J Nanobiotechnology

Carbon dot-based treatment for bacterial pneumonia by promoting a PI3K-mediated M1 polarization of macrophages

Authors - Xuan Jiang
View Article
||
New chat

Able™

正在加载，请稍候...