Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 13723-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13723-1-AP
WB result of VPS34 antibody (13723-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 transfected Jurkat cells.
WB analysis of mouse testis using 13723-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 13723-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 13723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13723-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 13723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 13723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 13723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

VPS34 Polyclonal antibody

VPS34 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13723-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

PIK3C3, EC:2.7.1.137, hVps34, PI 3 Kinase Class 3, PI 3-kinase p100

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, mouse lung tissue, Jurkat cells
Positive IHC detected in	mouse skeletal muscle tissue, human stomach cancer tissue, mouse skeletal muscle tissue, mouse stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IP	See 1 publications below

Product Information

13723-1-AP targets VPS34 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	phosphoinositide-3-kinase, class 3
Calculated molecular weight	887 aa, 100 kDa
Observed molecular weight	102 kDa
GenBank accession number	BC053651
Gene Symbol	VPS34
Gene ID (NCBI)	5289
RRID	AB_10646474
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NEB9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

Protocols

Product Specific Protocols
IHC protocol for VPS34 antibody 13723-1-AP	Download protocol
WB protocol for VPS34 antibody 13723-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IP	Cancer Lett Nutrient deprivation promotes bladder cancer metastasis through Beclin-1 deacetylation-mediated autophagy activation. Authors - Yan Sun View Article
human	WB	Nat Struct Mol Biol Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging Authors - Rui Guo View Article
mouse	WB	Front Pharmacol Integrated Network Pharmacology and Lipidomics to Reveal the Inhibitory Effect of Qingfei Oral Liquid on Excessive Autophagy in RSV-Induced Lung Inflammation. Authors - Lili Lin View Article
pig	WB	J Virol Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy Authors - Yunhang Zhang KD Validated View Article
mouse	WB	Psychopharmacology (Berl) 3-Methyladenine administration alleviates cognitive and memory dysfunction in attention-deficit/hyperactivity disorder by modulating autophagy. Authors - Ge-Ge Lv View Article
rat	WB	J Cardiovasc Transl Res The Protective Mechanism of Dexmedetomidine in Regulating Atg14L-Beclin1-Vps34 Complex Against Myocardial Ischemia-Reperfusion Injury. Authors - Yanna Li View Article

Validation Data Gallery

WB analysis of HeLa using 13723-1-AP

WB analysis of Jurkat using 13723-1-AP

WB analysis of mouse testis using 13723-1-AP

WB analysis of mouse lung using 13723-1-AP

IHC staining of mouse skeletal muscle using 13723-1-AP

IHC staining of human stomach cancer using 13723-1-AP

IHC staining of mouse stomach using 13723-1-AP

IHC staining of mouse skeletal muscle using 13723-1-AP

IHC staining of mouse skeletal muscle using 13723-1-AP

VPS34 Polyclonal antibody

VPS34 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Nutrient deprivation promotes bladder cancer metastasis through Beclin-1 deacetylation-mediated autophagy activation.

Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging

Integrated Network Pharmacology and Lipidomics to Reveal the Inhibitory Effect of Qingfei Oral Liquid on Excessive Autophagy in RSV-Induced Lung Inflammation.

Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy

3-Methyladenine administration alleviates cognitive and memory dysfunction in attention-deficit/hyperactivity disorder by modulating autophagy.

The Protective Mechanism of Dexmedetomidine in Regulating Atg14L-Beclin1-Vps34 Complex Against Myocardial Ischemia-Reperfusion Injury.

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