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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis using 19427-1-AP (same clone as 19427-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis of human testis using 19427-1-AP (same clone as 19427-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis of mouse eye using 19427-1-AP (same clone as 19427-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human uterus tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis of human uterus using 19427-1-AP (same clone as 19427-1-PBS)

    human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PIH1D1 Polyclonal antibody (19427-1-AP)

    IP experiment of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PIH1D1 Polyclonal antibody (19427-1-AP)

    IF Staining of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

PIH1D1 Polyclonal antibody, PBS Only

PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19427-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19427-1-PBS targets PIH1D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13759

Product name: Recombinant human PIH1D1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC001108

Sequence: MANPKLLGLELSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEIDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLLVPS

相同性解析による交差性が予測される生物種
Full Name PIH1 domain containing 1
Calculated molecular weight 290 aa, 32 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC001108
Gene Symbol PIH1D1
Gene ID (NCBI) 55011
RRIDAB_10640423
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWS0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.

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