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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIGZ Polyclonal antibody

PIGZ Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17384-1-AP

Synonyms

GPI mannosyltransferase 4, GPI mannosyltransferase IV, GPI MT IV, hSMP3, PIG Z, PIGZ, SMP3, SMP3 homolog

Validation Data Gallery

Applications:
  • WB analysis of human brain using 17384-1-AP

    WB analysis of human brain using 17384-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 17384-1-AP

    WB analysis of HEK-293 using 17384-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 17384-1-AP

    WB analysis of human testis using 17384-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17384-1-AP

    IHC staining of human colon using 17384-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17384-1-AP (PIGZ antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 17384-1-AP

    IHC staining of human colon using 17384-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17384-1-AP (PIGZ antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 17384-1-AP

    IF Staining of HepG2 using 17384-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17384-1-AP (PIGZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, human testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17384-1-AP is PIGZ Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIGZ fusion protein Ag11259 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol glycan anchor biosynthesis, class Z
Calculated molecular weight 579 aa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC044640
Gene symbol PIGZ
Gene ID (NCBI) 80235
RRIDAB_10697833
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PIGZ antibody 17384-1-APDownload protocol
IHC protocol for PIGZ antibody 17384-1-APDownload protocol
IF protocol for PIGZ antibody 17384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Phenomics

A Genome-Wide Association Study for Susceptibility to Axial Length in Highly Myopic Eyes

Authors - Qiang Lu
View Article
humanWB

J Biol Chem

Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein (PrP) into pro-PrP via the ATF6-miR449c-5p-PIGV axis

Authors - JingFeng Li
View Article