Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293K cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17120-1-AP targets PIGX in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10808 Product name: Recombinant human PIGX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC022542 Sequence: MCSEIILRQEVLKDGFHRDLLIKVKFGESIEDLHTCRLLIKQDIPAGLYVDPYELASLRERNITEAVMVSENFDIEAPNYLSKESEVLIYARRDSQCIDCFQAFLPVHCRYHRPHSEDGEASIVVNNPDLLMFCDQEFPILKCWAHSEVAAPCALDNEDICQWNKMKYKSVYKNVILQVPVGLTVHTSLVCSVTLLITILCSTLILVAVFKYGHFSL 相同性解析による交差性が予測される生物種 |
| Full Name | phosphatidylinositol glycan anchor biosynthesis, class X |
| Calculated molecular weight | 217aa,25 kDa; 258aa,29 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC022542 |
| Gene Symbol | PIGX |
| Gene ID (NCBI) | 54965 |
| RRID | AB_3669260 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TBF5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIGX encodes a 258-amino acid ER-resident type I transmembrane protein with a large lumenal domain. It acts by stabilizing the mannosyltransferase PIGM. PIGX might function as a cyclic dimeric GMP phosphodiesterase.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PIGX antibody 17120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |