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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PIGS Polyclonal antibody (18334-1-AP)

    WB analysis of HeLa using 18334-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PIGS Polyclonal antibody (18334-1-AP)

    WB analysis of PC-3 using 18334-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PIGS Polyclonal antibody (18334-1-AP)

    WB analysis of HepG2 using 18334-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PIGS Polyclonal antibody (18334-1-AP)

    WB analysis of A549 using 18334-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PIGS Polyclonal antibody (18334-1-AP)

    WB analysis of mouse liver using 18334-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PIGS Polyclonal antibody (18334-1-AP)

    IF Staining of HepG2 using 18334-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIGS antibody (18334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PIGS Polyclonal antibody

PIGS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18334-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

UNQ1873/PRO4316, Phosphatidylinositol-glycan biosynthesis class S protein, GPI transamidase component PIG-S

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, mouse liver tissue, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

18334-1-AP targets PIGS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12950

Product name: Recombinant human PIGS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 313-555 aa of BC001319

Sequence: SSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWRKPEKTD

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol glycan anchor biosynthesis, class S
Calculated molecular weight 62 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC001319
Gene Symbol PIGS
Gene ID (NCBI) 94005
RRIDAB_2878532
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96S52
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIGS, also named as GPI transamidase component PIG-S or PRO4316, is a 555 amino acid protein, which belongs to the PIGS family. PIGS localizes in the endoplasmic reticulum membrane. PIGS is a component of the GPI transamidase complex. It is essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.

Protocols

Product Specific Protocols
IF protocol for PIGS antibody 18334-1-APDownload protocol
WB protocol for PIGS antibody 18334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.

Authors - Takahiro Nakane
View Article
human,mouseWB

Nat Commun

Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis

Authors - Xiaoqiong Wei
View Article
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