Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 16369-1-AP (same clone as 16369-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
WB analysis using 16369-1-AP (same clone as 16369-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 16369-1-AP (same clone as 16369-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 16369-1-AP (same clone as 16369-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16369-1-AP (PIGO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16369-1-AP (PIGO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16369-1-AP (PIGO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 16369-1-AP (same clone as 16369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 16369-1-AP (same clone as 16369-1-PBS)
Immunofluorescent analysis of MCF-7 cells, using PIGO antibody 16369-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16369-1-PBS in a different storage buffer formulation.

PIGO Polyclonal antibody, PBS Only

PIGO Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16369-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.-.-.-, Phosphatidylinositol-glycan biosynthesis class O protein, PIG O, PIG-O, RP11 182N22.4

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16369-1-PBS targets PIGO in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9232 Product name: Recombinant human PIGO protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 867-1089 aa of BC013987 Sequence: FLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQR 相同性解析による交差性が予測される生物種
Full Name	phosphatidylinositol glycan anchor biosynthesis, class O
Calculated molecular weight	1089aa,119 kDa; 454aa,50 kDa
Observed molecular weight	74 kDa
GenBank accession number	BC013987
Gene Symbol	PIGO
Gene ID (NCBI)	84720
RRID	AB_2164806
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TEQ8
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

PIGO (Phosphatidylinositol-glycan biosynthesis class O protein) is an enzyme crucial for attaching GPI anchors to proteins in the endoplasmic reticulum. PIGO encodes a 1,089 amino acid protein, that is involved in GPI biosynthesis (PMID: 22683086).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 16369-1-AP (same clone as 16369-1-PBS)

WB analysis using 16369-1-AP (same clone as 16369-1-PBS)

WB analysis of COLO 320 using 16369-1-AP (same clone as 16369-1-PBS)

WB analysis of A431 using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human kidney using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human liver using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human spleen using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human spleen using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human kidney using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human kidney using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human testis using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human testis using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human skin using 16369-1-AP (same clone as 16369-1-PBS)

IHC staining of human skin using 16369-1-AP (same clone as 16369-1-PBS)

IF Staining of MCF-7 using 16369-1-AP (same clone as 16369-1-PBS)

PIGO Polyclonal antibody, PBS Only

PIGO Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™