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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PIGA Polyclonal antibody (13679-1-AP)

    WB analysis of HeLa using 13679-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PIGA Polyclonal antibody (13679-1-AP)

    WB analysis of HEK-293 using 13679-1-AP

    WB result of PIGA antibody (13679-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIGA transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using PIGA Polyclonal antibody (13679-1-AP)

    WB analysis of HeLa using 13679-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of mouse lung using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of mouse lung using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of rat liver using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of rat liver using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

PIGA Polyclonal antibody

PIGA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13679-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

GlcNAc PI synthesis protein, GPI3, PIG A, PIGA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse lung tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13679-1-AP targets PIGA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4530

Product name: Recombinant human PIGA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-421 aa of BC038236

Sequence: MACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQSTATTLFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHDRVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVGGIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDRVSVEAVLPMDKRLDRLISHCG

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol glycan anchor biosynthesis, class A
Calculated molecular weight 484 aa, 54 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC038236
Gene Symbol PIGA
Gene ID (NCBI) 5277
RRIDAB_2164656
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP37287
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PIGA antibody 13679-1-APDownload protocol
WB protocol for PIGA antibody 13679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int Immunopharmacol

Integrative analysis of scRNA-seq and bulk RNA-seq to identify lactylation-related gene signatures in lung ischemia-reperfusion injury after lung transplantation

Authors - Jiameng Gao
View Article
mouseIF,WB

Hum Mol Genet

CNS glycosylphosphatidylinositol deficiency results in delayed white matter development, ataxia and premature death in a novel mouse model.

Authors - Marshall Lukacs
  • KO Validated
View Article
MouseIHC

Front Genet

Machine learning potential predictor of idiopathic pulmonary fibrosis

Authors - Chenchun Ding
View Article
mouseWB,IHC

bioRxiv

Gene replacement therapy for Piga GPI-anchor deficiency in the developing nervous system.

Authors - Jennifer L. Watts
  • KO Validated
View Article
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