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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIBF1 Polyclonal antibody (14413-1-AP)

    WB analysis using 14413-1-AP (same clone as 14413-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PIBF1 Polyclonal antibody (14413-1-AP)

    WB analysis of HeLa using 14413-1-AP (same clone as 14413-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PIBF1 Polyclonal antibody (14413-1-AP)

    WB analysis of MCF-7 using 14413-1-AP (same clone as 14413-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PIBF1 Polyclonal antibody (14413-1-AP)

    WB analysis of K-562 using 14413-1-AP (same clone as 14413-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PIBF1 Polyclonal antibody (14413-1-AP)

    IP experiment of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

    IP result of anti-PIBF1 (IP:14413-1-AP, 4ug; Detection:14413-1-AP 1:1000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PIBF1 Polyclonal antibody (14413-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 14413-1-AP (same clone as 14413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14413-1-AP (PIBF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PIBF1 Polyclonal antibody (14413-1-AP)

    IF Staining of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PIBF1 antibody (14413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PIBF1 Polyclonal antibody, PBS Only

PIBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14413-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14413-1-PBS targets PIBF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5755

Product name: Recombinant human PIBF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 452-757 aa of BC051911

Sequence: KVTEFLHQSKLKSFESERVQLLQEETARNLTQCQLECEKYQKKLEVLTKEFYSLQASSEKRITELQAQNSEHQARLDIYEKLEKELDEIIMQTAEIENEDEAERVLFSYGYGANVPTTAKRRLKQSVHLARRVLQLEKQNSLILKDLEHRKDQVTQLSQELDRANSLLNQTQQPYRYLIESVRQRDSKIDSLTESIAQLEKDVSNLNKEKSALLQTKNQMALDLEQLLNHREELAAMKQILVKMHSKHSENSLLLTKTEPKHVTENQKSKTLNVPKEHEDNIFTPKPTLFTKKEAPEWSKKQKMKT

相同性解析による交差性が予測される生物種
Full Name progesterone immunomodulatory binding factor 1
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC051911
Gene Symbol PIBF1
Gene ID (NCBI) 10464
RRIDAB_10858916
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXW3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.

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