Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
Product Information
13486-1-AP targets PIAS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4304 Product name: Recombinant human PIAS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 277-628 aa of BC030556 Sequence: VRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILSSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSPVQGGDPSENKKKVEVIDLTIESSSDEEDLPPTKKHCSVTSAAIPALPGSKGVLTSGHQPSSVLRSPAMGTLGGDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDALGHFFQYRGTPSHFLGPLAPTLGSSHCSATPAPPPGRVSSIVAPGGALREGHGGPLPSGPSLTGCRSDIISLD 相同性解析による交差性が予測される生物種 |
| Full Name | protein inhibitor of activated STAT, 3 |
| Calculated molecular weight | 628 aa, 68 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC030556 |
| Gene Symbol | PIAS3 |
| Gene ID (NCBI) | 10401 |
| RRID | AB_2164224 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6X2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIAS3(E3 SUMO-protein ligase PIAS3) is a protein of 583 amino acid, has a molecular mass of 68 kDa and belongs to a family of proteins that includes several other members(PMID:11060035). PIAS3 is an inhibitor of activated STAT3 and binds microphthalmia-associated transcription factor, which is a key DNA-binding protein, in rat basophilic leukemia cells and mouse melanocytes(PMID:11709556). The PIAS3 antibody detects PIAS3 (68 kDa) as well as its sumoylated form (85 kDa) in some normal mouse tissues, breast and brain tumor cell lines(PMID:20371673).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PIAS3 antibody 13486-1-AP | Download protocol |
| IHC protocol for PIAS3 antibody 13486-1-AP | Download protocol |
| WB protocol for PIAS3 antibody 13486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting ZMIZ1 induces differentiation in acute myeloid leukemia via chromatin remodeling. | ||
Pharmacol Res PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma
| ||
Int J Biol Macromol Hyaluronic acid-modified PtPdCo-CQ nanocatalyst with triple enzyme-like activities regulates macrophage polarization and autophagy levels for the treatment of rheumatoid arthritis | ||
Free Radic Biol Med PIAS3 acts as a zinc sensor under zinc deficiency and plays an important role in myocardial ischemia/reperfusion injury | ||
Int J Mol Sci Resveratrol Enhances Temozolomide Efficacy in Glioblastoma Cells through Downregulated MGMT and Negative Regulators-Related STAT3 Inactivation | ||
Mol Med Rep Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose |