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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI4KA Recombinant monoclonal antibody (82938-1-RR)

    WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PI4KA Recombinant monoclonal antibody (82938-1-RR)

    WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PI4KA Recombinant monoclonal antibody (82938-1-RR)

    IHC staining of human placenta using 82938-1-RR (same clone as 82938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82938-1-RR (PI4KA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PI4KA Recombinant monoclonal antibody (82938-1-RR)

    IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PI4KA Recombinant monoclonal antibody (82938-1-RR)

    FC experiment of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PI4KA Recombinant antibody (82938-1-RR, Clone:230057G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82938-1-RR

    Affinity and Kinetic Characterization of 82938-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82938-1-RR against Human PI4KA were performed. The affinity constant is below 1 pM.

PI4KA Recombinant monoclonal antibody, PBS Only

PI4KA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82938-1-PBS
Clone No.230057G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230057G2, EC:2.7.1.67, Phosphatidylinositol 4-kinase alpha, Phosphatidylinositol 4-Kinase III alpha, PI4K-alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82938-1-PBS targets PI4KA in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3062

Product name: Recombinant human PI4KA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1745-2044 aa of BC018120

Sequence: YLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol 4-kinase, catalytic, alpha
Calculated molecular weight 2044 aa, 231 kDa
Observed molecular weight 231 kDa
GenBank accession numberBC018120
Gene Symbol PI4KA
Gene ID (NCBI) 5297
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP42356
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

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