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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PI4K2B Polyclonal antibody (15074-1-AP)

    WB analysis of HepG2 using 15074-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15074-1-AP (PI4K2B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PI4K2B Polyclonal antibody (15074-1-AP)

    WB analysis of mouse kidney using 15074-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15074-1-AP (PI4K2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PI4K2B Polyclonal antibody (15074-1-AP)

    IHC staining of human liver using 15074-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15074-1-AP (PI4K2B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PI4K2B Polyclonal antibody (15074-1-AP)

    IHC staining of human liver using 15074-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15074-1-AP (PI4K2B antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PI4K2B Polyclonal antibody (15074-1-AP)

    IF Staining of HepG2 using 15074-1-AP

    Immunofluorescent analysis of HepG2 cells, using PI4K2B antibody 15074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PI4K2B Polyclonal antibody (15074-1-AP)

    IF Staining of HepG2 using 15074-1-AP

    Immunofluorescent analysis of HepG2 cells, using PI4K2B antibody 15074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PI4K2B Polyclonal antibody

PI4K2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15074-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PI4KII-BETA, PI4KIIB, PI4KII BETA, Phosphatidylinositol 4-kinase type II-beta, Phosphatidylinositol 4-kinase type 2-beta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, mouse kidney tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15074-1-AP targets PI4K2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7120

Product name: Recombinant human PI4K2B protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 132-481 aa of BC051749

Sequence: GSYFVKDPKRKIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKIGSFQLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDEEFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol 4-kinase type 2 beta
Calculated molecular weight 55 kDa
Observed molecular weight 50-54 kDa
GenBank accession numberBC051749
Gene Symbol PI4K2B
Gene ID (NCBI) 55300
RRIDAB_2163903
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PI4K2B antibody 15074-1-APDownload protocol
IHC protocol for PI4K2B antibody 15074-1-APDownload protocol
WB protocol for PI4K2B antibody 15074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

FKBP51 plays an essential role in Akt ubiquitination that requires Hsp90 and PHLPP

Authors - Martina Tufano
View Article
humanIF

EMBO J

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Authors - Zhengming Wu
View Article
humanIHC

BMC Med Genomics

The identification of a two-gene prognostic model based on cisplatin resistance-related ceRNA network in small cell lung cancer

Authors - Yani Zhang
View Article
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