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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    WB analysis of MCF-7 using 27035-1-AP (same clone as 27035-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27035-1-AP (PI 3 Kinase p55 gamma antibody at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    WB analysis of HEK-293 using 27035-1-AP (same clone as 27035-1-PBS)

    WB result of PI 3 Kinase p55 gamma antibody (27035-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI 3 Kinase p55 gamma transfected HEK-293 cells. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    WB analysis of HUVEC using 27035-1-AP (same clone as 27035-1-PBS)

    WB result of PIK3R3 antibody (27035-1-AP) on whole cell extract from HUVEC targeted by shRNAs (#1, #2) or a control shRNA (NT). (Data from Dr. Fuqiang Geng, Weill Cornell Medical College). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of mouse testis using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of mouse kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PI3 Kinase p55 Gamma Polyclonal antibody, PBS Only

PI3 Kinase p55 Gamma Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27035-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27035-1-PBS targets PI3 Kinase p55 Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25884

Product name: Recombinant human PIK3R3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 312-382 aa of BC021622

Sequence: HLVWLNHKGVRQKRLNVWLGIKNEDAAENYFINEEDENLPHYDEKTWFVEDINRVQAEDLLYGKPDGAFLI

相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Calculated molecular weight 85 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC021622
Gene Symbol PI3 Kinase p55 Gamma
Gene ID (NCBI) 8503
RRIDAB_2880730
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92569
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.

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