Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human skin cancer tissue, human oesophagus tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 4 publications below |
Product Information
15963-1-AP targets Elafin/Skalp in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8735 Product name: Recombinant human PI3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-117 aa of BC010952 Sequence: AAVTGVPVKGQDTVKGRVPFNGQDPVKGQVSVKGQDKVKAQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ 相同性解析による交差性が予測される生物種 |
| Full Name | peptidase inhibitor 3, skin-derived |
| Calculated molecular weight | 117 aa, 12 kDa |
| GenBank accession number | BC010952 |
| Gene Symbol | Elafin/Skalp |
| Gene ID (NCBI) | 5266 |
| RRID | AB_2878201 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19957 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Elafin/Skalp antibody 15963-1-AP | Download protocol |
| IHC protocol for Elafin/Skalp antibody 15963-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ther Adv Med Oncol Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma. | ||
Anim Biosci Spatiotemporal expression and regulation of peptidase inhibitor 3 and secretory leukocyte protease inhibitor at the maternal-fetal interface in pigs | ||
Int J Gen Med FAM3B Serves as a Biomarker for the Development and Malignancy of Oral Lichen Planus. | ||
Front Endocrinol (Lausanne) Elafin is related to immune infiltration and could predict the poor prognosis in ovarian cancer |
