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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI16 Polyclonal antibody (12267-1-AP)

    WB analysis using 12267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PI16 Polyclonal antibody (12267-1-AP)

    WB analysis using 12267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using PI16 Polyclonal antibody (12267-1-AP)

    WB analysis of A2780 using 12267-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PI16 Polyclonal antibody (12267-1-AP)

    WB analysis of COLO 320 using 12267-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of human pancreas cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of human pancreas cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of mouse testis using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of mouse testis using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12267-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells, A2780 cells, COLO 320 cells, A549 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human pancreas cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12267-1-AP targets PI16 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2925

Product name: Recombinant human PI16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC022399

Sequence: MHGSCSFLMLLLPLLLLLVATTGPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPSFRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSMATEAPPCVTTEVPSILAAHSLPSL

相同性解析による交差性が予測される生物種
Full Name peptidase inhibitor 16
Calculated molecular weight 463 aa, 49 kDa
Observed molecular weight49 kDa
GenBank accession numberBC022399
Gene Symbol PI16
Gene ID (NCBI) 221476
RRIDAB_10697651
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).

Protocols

Product Specific Protocols
WB protocol for PI16 antibody 12267-1-APDownload protocol
IHC protocol for PI16 antibody 12267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IHC,CoIP

Cell Mol Biol Lett

Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO

Authors - Xiangqin Kuang
  • KD Validated
View Article
humanIF

Clin Cancer Res

Single-cell transcriptomic landscape deciphers intratumoral heterogeneity and subtypes of acral and mucosal melanoma

Authors - Yunyan Li
View Article