Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25804-1-AP targets PHOX2A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22873 Product name: Recombinant human PHOX2A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 151-284 aa of BC041564 Sequence: AASAKGAAGAAGAKKGEARCSSEDDDSKESTCSPTPDSTASLPPPPAPGLASPRLSPSPLPVALGSGPGPGPGPQPLKGALWAGVAGGGGGGPGAGAAELLKAWQPAESGPGPFSGVLSSFHRKPGPALKTNLF 相同性解析による交差性が予測される生物種 |
| Full Name | paired-like homeobox 2a |
| Calculated molecular weight | 284 aa, 30 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC041564 |
| Gene Symbol | PHOX2A |
| Gene ID (NCBI) | 401 |
| RRID | AB_2880244 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHOX2A antibody 25804-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
