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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHLPP2 Polyclonal antibody (25244-1-AP)

    WB analysis using 25244-1-AP (same clone as 25244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PHLPP2 Polyclonal antibody (25244-1-AP)

    WB analysis of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    WB result of PHLPP2 antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPP2 transfected HeLa cells. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PHLPP2 Polyclonal antibody (25244-1-AP)

    IP experiment of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    IP result of anti-PHLPP2 (IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHLPP2 Polyclonal antibody (25244-1-AP)

    IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PHLPP2 Polyclonal antibody (25244-1-AP)

    IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PHLPP2 Polyclonal antibody (25244-1-AP)

    IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHLPP2 Polyclonal antibody (25244-1-AP)

    IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHLPP2 Polyclonal antibody (25244-1-AP)

    IF Staining of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPP2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PHLPP2 Polyclonal antibody, PBS Only

PHLPP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25244-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PHLPPL, EC:3.1.3.16, KIAA0931, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25244-1-PBS targets PHLPP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19000

Product name: Recombinant human PHLPPL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 575-781 aa of BC129927

Sequence: LDLQHNALTRLPDTLFSKALNLRYLNASANSLESLPSACTGEESLSMLQLLYLTNNLLTDQCIPVLVGHLHLRILHLANNQLQTFPASKLNKLEQLEELNLSGNKLKTIPTTIANCKRLHTLVAHSNNISIFPEILQLPQIQFVDLSCNDLTEILIPEALPATLQDLDLTGNTNLVLEHKTLDIFSHITTLKIDQKPLPTTDSTVTS

相同性解析による交差性が予測される生物種
Full Name PH domain and leucine rich repeat protein phosphatase-like
Calculated molecular weight 1323 aa, 147 kDa
Observed molecular weight 147 kDa
GenBank accession numberBC129927
Gene Symbol PHLPPL
Gene ID (NCBI) 23035
RRIDAB_2879985
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZVD8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PHLPP2 also named as PHLPPL is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.

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