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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHLDA1 Recombinant antibody (85783-1-RR)

    WB analysis using 85783-1-RR (same clone as 85783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85783-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PHLDA1 Recombinant antibody (85783-1-RR)

    WB analysis using 85783-1-RR (same clone as 85783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85783-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85783-1-RR

    Affinity and Kinetic Characterization of 85783-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85783-1-RR against Human PHLDA1 were performed. The affinity constant is below 1 pM.

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PHLDA1 Recombinant antibody, PBS Only

PHLDA1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85783-1-PBS
Clone No.243156B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85783-1-PBS targets PHLDA1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PHLDA1 fusion protein Ag13125 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology-like domain, family A, member 1
Calculated molecular weight 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC018929
Gene Symbol PHLDA1
Gene ID (NCBI) 22822
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WV24
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).