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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHIP Polyclonal antibody (20933-1-AP)

    WB analysis using 20933-1-AP (same clone as 20933-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using PHIP Polyclonal antibody (20933-1-AP)

    WB analysis of A375 using 20933-1-AP (same clone as 20933-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A375 cells using PHIP Polyclonal antibody (20933-1-AP)

    IP experiment of A375 using 20933-1-AP (same clone as 20933-1-PBS)

    IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PHIP Polyclonal antibody (20933-1-AP)

    IF Staining of MCF-7 using 20933-1-AP (same clone as 20933-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20933-1-PBS targets PHIP in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHIP fusion protein Ag14968 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain interacting protein
Calculated molecular weight 1821 aa, 207 kDa
Observed molecular weight 207 kDa
GenBank accession numberBC008909
Gene Symbol PHIP
Gene ID (NCBI) 55023
RRIDAB_10733522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.