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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHF8 Polyclonal antibody (29516-1-AP)

    WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 29516-1-AP (PHF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHF8 Polyclonal antibody (29516-1-AP)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHF8 Polyclonal antibody (29516-1-AP)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

PHF8 Polyclonal antibody, PBS Only

PHF8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29516-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29516-1-PBS targets PHF8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30701

Product name: Recombinant human PHF8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 786-948 aa of NM_001184896

Sequence: GQDRSSGSSSSGLGTVSNSPASQRTPGKRPIKRPAYWRTESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSN

相同性解析による交差性が予測される生物種
Full Name PHD finger protein 8
Calculated molecular weight 118 kDa
Observed molecular weight125-135 kDa
GenBank accession numberNM_001184896
Gene Symbol PHF8
Gene ID (NCBI) 23133
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPP1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Plant homeodomain finger protein 8 (PHF8) is a JmjC domain-containing protein. PHF8 is localized in the nucleolus and may regulate rRNA transcription. As a member of the KDM7 subfamily of enzymes, PHF8 is involved in demethylation of lysine residues in histones such as H3K27me2/1, H3K9me2/1 and H4K20me1. PHF8 has been reported to participate in cancer development and metastasis of various types of tumors(PMID: 33111613). PHF8 has 5 isoforms produced by alternative splicing(Uniprot).