Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells |
| Positive IHC detected in | human placenta tissue, human cervical cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse brain tissue, mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
51062-1-AP targets PHF6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2102 Product name: Recombinant human PHF6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC005994 Sequence: MSSSVEQKKGPTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDVQKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDKAQIREKPSQGIYMAYCRKHKKTAHNSEAADLEESFNEHELEPSSPKSKKKSRKGRPRKTNFKGLSEDTRSTSSHGTDEMESSSYRDRSPHRSSPSDTRPKCGFCHVGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMVCSFYICYATLHLICCFKFRVHPKFIQSSENLK 相同性解析による交差性が予測される生物種 |
| Full Name | PHD finger protein 6 |
| Calculated molecular weight | 365aa,41 kDa; 312aa,35 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC005994 |
| Gene Symbol | PHF6 |
| Gene ID (NCBI) | 84295 |
| RRID | AB_2165369 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PHF6 antibody 51062-1-AP | Download protocol |
| IHC protocol for PHF6 antibody 51062-1-AP | Download protocol |
| WB protocol for PHF6 antibody 51062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
