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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHF5A Monoclonal antibody (68196-1-Ig)

    WB analysis using 68196-1-Ig (same clone as 68196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68196-1-Ig (PHF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PHF5A Monoclonal antibody (68196-1-Ig)

    WB analysis of NIH/3T3 using 68196-1-Ig (same clone as 68196-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68196-1-Ig (PHF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68196-1-PBS in a different storage buffer formulation.

PHF5A Monoclonal antibody, PBS Only

PHF5A Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68196-1-PBS
Clone No.1G7E2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68196-1-PBS targets PHF5A in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7804

Product name: Recombinant human INI-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-110 aa of BC007321

Sequence: MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR

相同性解析による交差性が予測される生物種
Full Name PHD finger protein 5A
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC007321
Gene Symbol PHF5A
Gene ID (NCBI) 84844
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ7RTV0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.