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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHF24 Polyclonal antibody

PHF24 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27959-1-AP

Synonyms

DKFZp434I2112, GINIP, KIAA1045, PHF24, Protein KIAA1045, RP11 392A14.4

Validation Data Gallery

Applications:
  • IHC staining of mouse brain using 27959-1-AP

    IHC staining of mouse brain using 27959-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27959-1-AP (PHF24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27959-1-AP

    IHC staining of mouse brain using 27959-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27959-1-AP (PHF24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27959-1-AP

    IF Staining of mouse brain using 27959-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PHF24 antibody (27959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 27959-1-AP

    IF Staining of mouse brain using 27959-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PHF24 antibody (27959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27959-1-AP targets PHF24 in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHF24 fusion protein Ag27492 相同性解析による交差性が予測される生物種
Full Name KIAA1045
Calculated molecular weight 45 aa
GenBank accession numberNM_001304333
Gene symbol KIAA1045
Gene ID (NCBI) 23349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PHF24 antibody 27959-1-APDownload protocol
IF protocol for PHF24 antibody 27959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols