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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis of HepG2 using 28472-1-AP (same clone as 28472-1-PBS)

    WB result of PHF17 antibody (28472-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF17 transfected HepG2 cells. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis of A549 using 28472-1-AP (same clone as 28472-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHF17 Polyclonal antibody (28472-1-AP)

    IF Staining of HeLa using 28472-1-AP (same clone as 28472-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF17 antibody (28472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PHF17 Polyclonal antibody, PBS Only

PHF17 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28472-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Jade family PHD finger protein 1, JADE1, PHF 17, Protein Jade-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28472-1-PBS targets PHF17 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29675

Product name: Recombinant human PHF17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 360-450 aa of BC032376

Sequence: KFKSYCPKHSSHRKPEESLGKGAAQENGAPECSPRNPLEPFASLEQNREEAHRVSVRKQKLQQLEDEFYTFVNLLDVARALRLPEEVVDFL

相同性解析による交差性が予測される生物種
Full Name PHD finger protein 17
Calculated molecular weight 96 kDa
Observed molecular weight65 kDa, 100-110 kDa
GenBank accession numberBC032376
Gene Symbol PHF17
Gene ID (NCBI) 79960
RRIDAB_2918168
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IE81
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PHF17, also named as JADE1 and KIAA1807, belongs to the JADE family. PHF17 codes for the Jade-1 protein. This protein interacts with histone acetyltransferase HBO1 and promotes histone acetylation in chromatin to regulate DNA replication and gene transcription. PHF17 is mostly expressed in the small intestine, prostate, mammary gland and kidney. PHF17 is involved in mitosis, meiosis and tumor suppression (PMID: 28134766). PHF17 has 3 isoforms with the molecular mass of 58 kDa and 94-96 kDa. With modification, the MW of PHF17 may be migrated to about 65 kDa and 100-110 kDa.

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