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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PHF12 Polyclonal antibody (24485-1-AP)

    WB analysis of mouse brain using 24485-1-AP (same clone as 24485-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24485-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PHF12 Polyclonal antibody (24485-1-AP)

    WB analysis of mouse brain using 24485-1-AP (same clone as 24485-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24485-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using PHF12 Polyclonal antibody (24485-1-AP)

    WB analysis of mouse lung using 24485-1-AP (same clone as 24485-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24485-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PHF12 Polyclonal antibody (24485-1-AP)

    IP experiment of mouse brain using 24485-1-AP (same clone as 24485-1-PBS)

    IP result of anti-PHF12 (IP:24485-1-AP, 4ug; Detection:24485-1-AP 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 24485-1-PBS in a different storage buffer formulation.

PHF12 Polyclonal antibody, PBS Only

PHF12 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 24485-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24485-1-PBS targets PHF12 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18471

Product name: Recombinant human PHF12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-302 aa of BC121043

Sequence: MWEKMETKTIVYDLDTSGGLMEQIQALLAPPKTDEAEKRSRKPEKEPRRSGRATNHDSCDSCKEGGDLLCCDHCPAAFHLQCCNPPLSEEMLPPGEWMCHRCTVRRKKREQKKELGHVNGLVDKSGKRTTSPSSDTDLLDRSASKTELKAIAHARILERRASRPGTPTSSASTETPTSEQNDVDEDIIDVDEEPVAAEPDYVQPQLRRPFELLIAAAMERNPTQFQLPNELTCTTALPGSSKRRRKEETTGKNVKKTQHELDHNGLVPLPVKVCFTCNRSCRVAPLIQCDYCPLLFHMDCLE

相同性解析による交差性が予測される生物種
Full Name PHD finger protein 12
Calculated molecular weight 1004 aa, 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC121043
Gene Symbol PHF12
Gene ID (NCBI) 57649
RRIDAB_2879566
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QT6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Plant homeodomain [PHD] zinc finger protein 12 (PHF12), also known Pf1, is a member of the PHD zinc finger family of proteins. Pf1 associates with a chromatin-interacting protein complex comprised of MRG15, Sin3B, and histone deacetylase 1 (HDAC1) that functions as a transcriptional modulator.

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