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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHC2 Polyclonal antibody

PHC2 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12867-1-AP

Synonyms

EDR2, HPH2, PH2, PHC2, Polyhomeotic like protein 2

Validation Data Gallery

Applications:
  • WB analysis of A375 using 12867-1-AP

    WB analysis of A375 using 12867-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 12867-1-AP

    WB analysis of A549 using 12867-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 12867-1-AP

    IP experiment of HL-60 using 12867-1-AP

    IP result of anti-PHC2 (IP:12867-1-AP, 4ug; Detection:12867-1-AP 1:500) with HL-60 cells lysate 4000 ug.

  • IF Staining of A375 using 12867-1-AP

    IF Staining of A375 using 12867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12867-1-AP (PHC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, A549 cells
Positive IP detected inHL-60 cells
Positive IF detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy PHC2 Ready-To-Use IHC Kit. PHC2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12867-1-AP targets PHC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHC2 fusion protein Ag3507 相同性解析による交差性が予測される生物種
Full Name polyhomeotic homolog 2 (Drosophila)
Calculated molecular weight 858 aa, 91 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC028396
Gene symbol PHC2
Gene ID (NCBI) 1912
RRIDAB_2877890
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHC2 antibody 12867-1-APDownload protocol
IF protocol for PHC2 antibody 12867-1-APDownload protocol
IP protocol for PHC2 antibody 12867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols