Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 12295-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12295-1-AP
WB result of Prohibitin 2 antibody (12295-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.
IP experiment of HeLa using 12295-1-AP
IP result of anti-Prohibitin 2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.
IP experiment of mouse brain using 12295-1-AP
IP result of anti-Prohibitin 2 (IP:12295-1-AP, 4ug; Detection:12295-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human heart using 12295-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 12295-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human pancreas using 12295-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 12295-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 12295-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Prohibitin 2 antibody (12295-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 12295-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 12295-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Prohibitin 2 (12295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Prohibitin 2 Polyclonal antibody

Prohibitin 2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12295-1-AP

Publications(69)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Electrochemical Immunosensor

Synonyms

PHB2, BAP, B-cell receptor-associated protein BAP37, D prohibitin, D-prohibitin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (69)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IP detected in	HeLa cells, mouse brain tissue
Positive IHC detected in	human heart tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 56 publications below
IHC	See 5 publications below
IF	See 13 publications below
IP	See 5 publications below
CoIP	See 4 publications below

Product Information

12295-1-AP targets Prohibitin 2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Electrochemical Immunosensor applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, rice
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2977 Product name: Recombinant human PHB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-299 aa of BC014766 Sequence: MAQNLKDLAGRLPAGPRGMGTALKLLLGAGAVAYGVRESVFTVEGGHRAIFFNRIGGVQQDTILAEGLHFRIPWFQYPIIYDIRARPRKISSPTGSKDLQMVNISLRVLSRPNAQELPSMYQRLGLDYEERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRELTERAKDFSLILDDVAITELSFSREYTAAVEAKQVAQQEAQRAQFLVEKAKQEQRQKIVQAEGEAEAAKMLGEALSKNPGYIKLRKIRAAQNISKTIATSQNRIYLTADNLVLNLQDESFTRGSDSLIKGKK 相同性解析による交差性が予測される生物種
Full Name	prohibitin 2
Calculated molecular weight	299 aa, 33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC014766
Gene Symbol	Prohibitin 2
Gene ID (NCBI)	11331
RRID	AB_2164779
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99623
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

Protocols

Product Specific Protocols
FC protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
IF protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
IHC protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
IP protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
WB protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE complex mediating autophagosome-lysosome fusion Authors - Fenglei Jian View Article
human	WB	Autophagy PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis. Authors - Chaojun Yan KD Validated View Article
mouse,human	WB,IF,CoIP	Autophagy Salmonella Typhimurium persistently infects host via its effector SseJ-induced PHB2-mediated mitophagy Authors - Dage Sun KD Validated View Article
mouse,human	WB	Autophagy BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains. Authors - Xiaoli Wu View Article
mouse	WB	Nat Commun Snhg18 regulates Yap subcellular localization to maintain bone homeostasis. Authors - Jie Huang View Article
rat	IF	Nat Commun Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Authors - Y Poitelon View Article

Validation Data Gallery

WB analysis using 12295-1-AP

WB analysis of HeLa using 12295-1-AP

IP experiment of HeLa using 12295-1-AP

IP experiment of mouse brain using 12295-1-AP

IHC staining of human heart using 12295-1-AP

IHC staining of human heart using 12295-1-AP

IHC staining of human pancreas using 12295-1-AP

IHC staining of human pancreas using 12295-1-AP

IF Staining of HepG2 using 12295-1-AP

IF Staining of HepG2 using 12295-1-AP

FC experiment of HeLa using 12295-1-AP

Prohibitin 2 Polyclonal antibody

Prohibitin 2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE complex mediating autophagosome-lysosome fusion

PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Salmonella Typhimurium persistently infects host via its effector SseJ-induced PHB2-mediated mitophagy

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.

Snhg18 regulates Yap subcellular localization to maintain bone homeostasis.

Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.

Able™

Able^™