Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 10787-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10787-1-AP (Prohibitin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10787-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10787-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10787-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10787-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 10787-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10787-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 10787-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10787-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 10787-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10787-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 10787-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10787-1-AP (Prohibitin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10787-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Featured Product
KD/KO Validated

Prohibitin Polyclonal antibody

Prohibitin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10787-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PHB, Prohibitin 1, PHB1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, human liver tissue, human brain tissue, NIH/3T3 cells, HeLa cells, Raji cells, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat kidney tissue
Positive IHC detected in	human liver cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 21 publications below
IHC	See 5 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

10787-1-AP targets Prohibitin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1240 Product name: Recombinant human Prohibitin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC013401 Sequence: MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ 相同性解析による交差性が予測される生物種
Full Name	prohibitin
Calculated molecular weight	30 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC013401
Gene Symbol	Prohibitin
Gene ID (NCBI)	5245
RRID	AB_2164476
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35232
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

Protocols

Product Specific Protocols
IF protocol for Prohibitin antibody 10787-1-AP	Download protocol
IHC protocol for Prohibitin antibody 10787-1-AP	Download protocol
WB protocol for Prohibitin antibody 10787-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts. Authors - Bei-Bei Chu View Article
human	WB	Autophagy PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis. Authors - Chaojun Yan View Article
mouse	WB	Cell Death Differ Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. Authors - H Li View Article
human	WB	Hum Mol Genet Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. Authors - McCray Brett A BA View Article
mouse	WB	Cell Mol Gastroenterol Hepatol DJ-1 deficiency in hepatocytes improves liver ischemia-reperfusion injury by enhancing mitophagy. Authors - Min Xu View Article
human	WB	Elife Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors. Authors - Sven Dennerlein View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10787-1-AP

WB analysis of HeLa using 10787-1-AP

WB analysis of Raji using 10787-1-AP

WB analysis of NIH/3T3 using 10787-1-AP

WB analysis of A431 using 10787-1-AP

WB analysis of human liver using 10787-1-AP

WB analysis of human brain using 10787-1-AP

IHC staining of human liver cancer using 10787-1-AP

IHC staining of human liver cancer using 10787-1-AP

IHC staining of human heart using 10787-1-AP

IHC staining of human heart using 10787-1-AP

IF Staining of HepG2 using 10787-1-AP

IF Staining of HeLa using 10787-1-AP

Prohibitin Polyclonal antibody

Prohibitin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.

PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

DJ-1 deficiency in hepatocytes improves liver ischemia-reperfusion injury by enhancing mitophagy.

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.