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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    WB analysis of mouse eye using 13408-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13408-1-AP (PHACTR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    WB analysis of human liver using 13408-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13408-1-AP (PHACTR4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PHACTR4 Polyclonal antibody (13408-1-AP)

    WB analysis of A431 using 13408-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13408-1-AP (PHACTR4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of human ovary cancer using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of human placenta using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of rat ovary using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of human ovary cancer using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of human placenta using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of mouse lung using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of mouse ovary using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of rat lung using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of rat ovary using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of human ovary cancer using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PHACTR4 Polyclonal antibody (13408-1-AP)

    IF Staining of A431 using 13408-1-AP

    Immunofluorescent analysis of A431 cells using 13408-1-AP (PHACTR4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PHACTR4 Polyclonal antibody

PHACTR4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13408-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PRO2963

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue, A431 cells, human liver tissue
Positive IHC detected inhuman ovary cancer tissue, human placenta tissue, rat lung tissue, rat ovary tissue, mouse ovary tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13408-1-AP targets PHACTR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4054

Product name: Recombinant human PHACTR4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 403-702 aa of BC029266

Sequence: FGEPHIPSRLPPLPLHIRIQQALTSPLPMTPILEGSHRAHSLLFENSDSFSEDSSTLGRTRSLPITIEMLKVPDDEEEEEQTCPSTFSEEMTPTSVIPKLPQCLREEEEKESDSDSEGPIQYRDEEDEDESYQSALANKVKRKDTLAMKLNHRPSEPELNLNSWPCKSKEEWNEIRHQIGNTLIRRLSQRPTPEELEQRNILQPKNEADRQAEKREIKRRLTRKLSQRPTVAELLARKILRFNEYVEVTDAQDYDRRADKPWTKLTPADKAAIRKELNEFKSSEMEVHEESKHFTRYHRP

相同性解析による交差性が予測される生物種
Full Name phosphatase and actin regulator 4
Calculated molecular weight 702 aa, 78 kDa
Observed molecular weight 71-78 kDa
GenBank accession numberBC029266
Gene Symbol PHACTR4
Gene ID (NCBI) 65979
RRIDAB_10638316
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZ21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PHACTR4 antibody 13408-1-APDownload protocol
IHC protocol for PHACTR4 antibody 13408-1-APDownload protocol
WB protocol for PHACTR4 antibody 13408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Phactr4 promotes oxidative stress and behavioral disorder caused by chronic stress via regulating PP1/GSK3-β pathway.

Authors - Tian Lan
  • KD Validated
View Article
mouseWB

Int J Biol Macromol

Targeting Phactr4 to rescue chronic stress-induced depression-like behavior in rats via regulating neuroinflammation and neuroplasticity

Authors - Ye Li
View Article
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