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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHACTR1 Polyclonal antibody

PHACTR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23446-1-AP

Synonyms

KIAA1733, PHACTR1, RPEL, RPEL1

Validation Data Gallery

Applications:
  • WB analysis using 23446-1-AP

    WB analysis using 23446-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23446-1-AP (PHACTR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 23446-1-AP

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 23446-1-AP

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

23446-1-AP targets PHACTR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHACTR1 fusion protein Ag20125 相同性解析による交差性が予測される生物種
Full Name phosphatase and actin regulator 1
Calculated molecular weight 580 aa, 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC101526
Gene symbol PHACTR1
Gene ID (NCBI) 221692
RRIDAB_2879281
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHACTR1 antibody 23446-1-APDownload protocol
IHC protocol for PHACTR1 antibody 23446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Pediatr

Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS

Authors - A Kaitlyn Victor
View Article