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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of MDA-MB-453s using 11491-1-AP (same clone as 11491-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of mouse testis using 11491-1-AP (same clone as 11491-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of HeLa using 11491-1-AP (same clone as 11491-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of mouse testis using 11491-1-AP (same clone as 11491-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PHACS Polyclonal antibody (11491-1-AP)

    IHC staining of human lymphoma using 11491-1-AP (same clone as 11491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11491-1-AP (PHACS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PHACS Polyclonal antibody (11491-1-AP)

    IHC staining of human lymphoma using 11491-1-AP (same clone as 11491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11491-1-AP (PHACS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11491-1-PBS in a different storage buffer formulation.

PHACS Polyclonal antibody, PBS Only

PHACS Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11491-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ACCS, 1-aminocyclopropane-1-carboxylate synthase-like protein 1, ACC synthase like protein 1, ACC synthase-like protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11491-1-PBS targets PHACS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2056

Product name: Recombinant human ACCS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 152-501 aa of BC020197

Sequence: YCKSPVPLRPENVVVLNGGASLFSALATVLCEAGEAFLIPTPYYGAITQHVCLYGNIRLAYVYLDSEVTGLDTRPFQLTVEKLEMALREAHSEGVKVKGLILISPQNPLGDVYSPEELQEYLVFAKRHRLHVIVDEVYMLSVFEKSVGYRSVLSLERLPDPQRTHVMWATSKDFGMSGLRFGTLYTENQDVATAVASLCRYHGLSGLVQYQMAQLLRDRDWINQVYLPENHARLKAAHTYVSEELRALGIPFLSRGAGFFIWVDLRKYLLKGTFEEEMLLWRRFLDNKVLLSFGKAFECKEPGWFRFVFSDQVHRLCLGMQRVQQVLAGKSQVAEDPRPSQSQEPSDQRR

相同性解析による交差性が予測される生物種
Full Name 1-aminocyclopropane-1-carboxylate synthase homolog (Arabidopsis)(non-functional)
Calculated molecular weight 501 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC020197
Gene Symbol PHACS
Gene ID (NCBI) 84680
RRIDAB_2222681
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QU6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACCS(1-aminocyclopropane-1-carboxylate synthase-like protein 1) is also named as PHACS and belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. The deduced 501-amino acid protein has a calculated molecular mass of 58 kD. It contains overlapping aminotransferase I and beta-eliminating lyase domains, and it shares structural similarity with aspartate aminotransferase, tyrosine aminotransferase, and enzymes that catalyze beta-elimination reactions on amino acids.

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