Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse heart tissue, K-562 cells, SH-SY5Y cells, Y79 cells |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | mouse heart tissue, human skeletal muscle tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
25081-1-AP targets PGP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18623 Product name: Recombinant human PGP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC157035 Sequence: MAAAEAGGDDARCVRLSAERAQALLADVDTLLFDCDGVLWRGETAVPGAPEALRALRARGKRLGFITNNSSKTRAAYAEKLRRLGFGGPAGPGASLEVFGTAYCTALYLRQRLAGAPAPKAYVLGSPALAAELEAVGVASVGVGPEPLQGEGPGDWLHAPLEPDVRAVVVGFDPHFSYMKLTKALRYLQQPGCLLVGTNMDNRLPLENGRFIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGINPERTVMVGDRLDTDILLGATCGLKTILTLTGVSTLGDVKNNQESDCVSKKKMVPDFYVDSIADLLPALQG 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoglycolate phosphatase |
| Calculated molecular weight | 321 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC157035 |
| Gene Symbol | PGP |
| Gene ID (NCBI) | 283871 |
| RRID | AB_2879888 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6NDG6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PGP(phosphoglycolate phosphatase) belongs to the HAD-like hydrolase superfamily. It is an essential intermediate in the biosynthetic pathway of cardiolipin and a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle. PGP catalyzes the formation of phosphatidylglycerol from phosphatidylglycerophosphate.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGP antibody 25081-1-AP | Download protocol |
| IHC protocol for PGP antibody 25081-1-AP | Download protocol |
| IF protocol for PGP antibody 25081-1-AP | Download protocol |
| IP protocol for PGP antibody 25081-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Biomater Systematic evaluation of multifunctional paclitaxel-loaded polymeric mixed micelles as a potential anticancer remedy to overcome multidrug resistance. | ||
Front Cell Dev Biol Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer. | ||
Chin Med The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters | ||
J Nanobiotechnology Combination of polythyleneimine regulating autophagy prodrug and Mdr1 siRNA for tumor multidrug resistance | ||
Eur J Med Chem Design, synthesis and biological evaluation of novel phenylfuran-bisamide derivatives as P-glycoprotein inhibitors against multidrug resistance in MCF-7/ADR cell | ||
J Peripher Nerv Syst Effect of age on metabolomic changes in a model of paclitaxel-induced peripheral neurotoxicity |