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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGM3 Polyclonal antibody

PGM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29686-1-AP

Synonyms

AGM1, DKFZp434B187, PAGM, PGM 3, PGM3, phosphoglucomutase 3

Validation Data Gallery

Applications:
  • WB analysis using 29686-1-AP

    WB analysis using 29686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29686-1-AP (PGM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 29686-1-AP

    IHC staining of human prostate hyperplasia using 29686-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 29686-1-AP

    IHC staining of human prostate hyperplasia using 29686-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29686-1-AP targets PGM3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGM3 fusion protein Ag30504 相同性解析による交差性が予測される生物種
Full Name phosphoglucomutase 3
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC001258
Gene symbol PGM3
Gene ID (NCBI) 5238
RRIDAB_2918339
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PGM3 antibody 29686-1-APDownload protocol
IHC protocol for PGM3 antibody 29686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols