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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGM3 Polyclonal antibody (29686-1-AP)

    WB analysis using 29686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29686-1-AP (PGM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PGM3 Polyclonal antibody (29686-1-AP)

    IHC staining of human prostate hyperplasia using 29686-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PGM3 Polyclonal antibody (29686-1-AP)

    IHC staining of human prostate hyperplasia using 29686-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 40x lens).

PGM3 Polyclonal antibody

PGM3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29686-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Synonyms

AGM1, DKFZp434B187, PAGM, PGM 3, PGM3, phosphoglucomutase 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29686-1-AP targets PGM3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30504

Product name: Recombinant human PGM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 380-542 aa of BC001258

Sequence: STAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF

相同性解析による交差性が予測される生物種
Full Name phosphoglucomutase 3
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC001258
Gene Symbol PGM3
Gene ID (NCBI) 5238
RRIDAB_2918339
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95394
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PGM3 antibody 29686-1-APDownload protocol
WB protocol for PGM3 antibody 29686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

PSMD11 stabilizes PGM3 by antagonizing Parkin to promote bladder cancer progression through energy metabolism reprogramming.

Authors - Yu Cheng
  • KD Validated
View Article
mouseWB,IHC

Biomaterials

Gut-confined fullerene reprograms lactate metabolism to Rescue hypoxia-induced exercise intolerance.

Authors - Shiliang Chen
View Article
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