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Validation Data Gallery

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  • Western Blot (WB) analysis of human urine sample using PGLYRP1 Recombinant antibody (85610-4-RR)

    WB analysis of human urine using 85610-4-RR (same clone as 85610-4-PBS)

    human urine sample were subjected to SDS PAGE followed by western blot with 85610-4-RR (PGLYRP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85610-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PGLYRP1 Recombinant antibody (85610-4-RR)

    IHC staining of mouse spleen using 85610-4-RR (same clone as 85610-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85610-4-RR (PGLYRP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85610-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PGLYRP1 Recombinant antibody (85610-4-RR)

    IHC staining of mouse spleen using 85610-4-RR (same clone as 85610-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85610-4-RR (PGLYRP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85610-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using PGLYRP1 Recombinant antibody (85610-4-RR)

    IF Staining of mouse spleen using 85610-4-RR (same clone as 85610-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using PGLYRP1 antibody (85610-4-RR, Clone: 243004F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85610-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using PGLYRP1 Recombinant antibody (85610-4-RR)

    IF Staining of mouse spleen using 85610-4-RR (same clone as 85610-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using PGLYRP1 antibody (85610-4-RR, Clone: 243004F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85610-4-PBS in a different storage buffer formulation.

PGLYRP1 Recombinant monoclonal antibody, PBS Only

PGLYRP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85610-4-PBS
Clone No.243004F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

PGLYRP, PGRP, PGRP S, PGRPS, PGRP-S

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85610-4-PBS targets PGLYRP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3122

Product name: Recombinant Human PGLYRP1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 22-196 aa of NM_005091.2

Sequence: QETEDPACCSPIVPRNEWKALASECAQHLSLPLRYVVVSHTAGSSCNTPASCQQQARNVQHYHMKTLGWCDVGYNFLIGEDGLVYEGRGWNFTGAHSGHLWNPMSIGISFMGNYMDRVPTPQAIRAAQGLLACGVAQGALRSNYVLKGHRDVQRTLSPGNQLYHLIQNWPHYRSP

相同性解析による交差性が予測される生物種
Full Name peptidoglycan recognition protein 1
Calculated molecular weight22 kDa
Observed molecular weight25 kDa
GenBank accession numberNM_005091.2
Gene Symbol PGLYRP1
Gene ID (NCBI) 8993
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75594
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Peptidoglycan recognition protein 1 (PGLYRP1, also known as PGRP-S) belongs to a family of evolutionary conserved innate immunity proteins, which in mammals also include PGLYRP2, PGLYRP3, and PGLYRP4 (PMID: 17275309; 20418257). PGLYRP1 is highly expressed in polymorphonuclear leukocytes and to a lower extent in many non-immune cells (PMID: 20418257). It recognizes bacterial peptidoglycan and functions in antibacterial immunity and inflammation (PMID: 20418257). PGLYRP1 has been identified as a ligand for TREM-1 (PMID: 25595774).

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