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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGLS Polyclonal antibody (30560-1-AP)

    WB analysis using 30560-1-AP (same clone as 30560-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30560-1-AP (PGLS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30560-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30560-1-PBS targets PGLS in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGLS fusion protein Ag30836 相同性解析による交差性が予測される生物種
Full Name 6-phosphogluconolactonase
Observed molecular weight28 kDa
GenBank accession numberBC014006
Gene Symbol PGLS
Gene ID (NCBI) 25796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95336
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

6-phosphogluconolactonase (PGLS) ,the key enzyme of pentose phosphate pathway, was aberrantly highly expressed in various cancers, including cervical cancer, breast cancer, glioblastomas, and hepatic cancer, etc.