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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGK2 Monoclonal antibody (68228-1-Ig)

    WB analysis using 68228-1-Ig (same clone as 68228-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PGK2 Monoclonal antibody (68228-1-Ig)

    WB analysis using 68228-1-Ig (same clone as 68228-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using PGK2 Monoclonal antibody (68228-1-Ig)

    WB analysis of HuH-7 using 68228-1-Ig (same clone as 68228-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using PGK2 Monoclonal antibody (68228-1-Ig)

    IHC staining of rat testis using 68228-1-Ig (same clone as 68228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68228-1-Ig (PGK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PGK2 Monoclonal antibody (68228-1-Ig)

    IHC staining of mouse testis using 68228-1-Ig (same clone as 68228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68228-1-Ig (PGK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PGK2 Monoclonal antibody (68228-1-Ig)

    IHC staining of mouse testis using 68228-1-Ig (same clone as 68228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68228-1-Ig (PGK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using PGK2 Monoclonal antibody (68228-1-Ig)

    IHC staining of rat testis using 68228-1-Ig (same clone as 68228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68228-1-Ig (PGK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PGK2 Monoclonal antibody (68228-1-Ig)

    IF Staining of mouse testis using 68228-1-Ig (same clone as 68228-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (68228-1-Ig, Clone: 3D4F2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using PGK2 Monoclonal antibody (68228-1-Ig)

    FC experiment of A549 using 68228-1-Ig (same clone as 68228-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human PGK2 (68228-1-Ig, Clone:3D4F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68228-1-PBS in a different storage buffer formulation.

PGK2 Monoclonal antibody, PBS Only

PGK2 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 68228-1-PBS
Clone No.3D4F2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

3D4F2, EC:2.7.2.3, PGKB, PGKPS

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68228-1-PBS targets PGK2 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag20631

Product name: Recombinant human PGK2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 242-360 aa of BC038843

Sequence: YTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIV

相同性解析による交差性が予測される生物種
Full Name phosphoglycerate kinase 2
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC038843
Gene Symbol PGK2
Gene ID (NCBI) 5232
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP07205
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

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