Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of mouse testis using 13686-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13686-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13686-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 13686-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13686-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13686-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 13686-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 13686-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of mouse testis using 13686-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 13686-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PGK2 Polyclonal antibody

PGK2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 13686-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

PGKPS, PGKB, EC:2.7.2.3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, HeLa cells, HepG2 cells, human heart tissue, human testis tissue, mouse brain tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

13686-1-AP targets PGK2 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4553 Product name: Recombinant human PGK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 242-360 aa of BC038843 Sequence: YTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIV 相同性解析による交差性が予測される生物種
Full Name	phosphoglycerate kinase 2
Calculated molecular weight	417 aa, 45 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC038843
Gene Symbol	PGK2
Gene ID (NCBI)	5232
RRID	AB_2161237
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07205
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

Protocols

Product Specific Protocols
IF protocol for PGK2 antibody 13686-1-AP	Download protocol
IHC protocol for PGK2 antibody 13686-1-AP	Download protocol
WB protocol for PGK2 antibody 13686-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Chemosphere Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice. Authors - Yongli Han View Article
mouse	WB	Cell Physiol Biochem Effect of Type I Diabetes on the Proteome of Mouse Oocytes. Authors - Guangjian Jiang View Article
sheep	WB	Theriogenology Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm. Authors - Xiaolei Yao View Article
mouse	WB	Nature A male germ-cell-specific ribosome controls male fertility Authors - Huiling Li View Article
human	WB	Cell Res Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate Authors - Yu-Qing Wu KD Validated View Article
mouse	WB,IHC,CoIP	Biomedicines The Role of Chorein Deficiency in Late Spermatogenesis Authors - Kaoru Arai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse testis using 13686-1-AP

WB analysis of HeLa using 13686-1-AP

WB analysis of mouse brain using 13686-1-AP

WB analysis of human testis using 13686-1-AP

WB analysis of human heart using 13686-1-AP

WB analysis of HepG2 using 13686-1-AP

IHC staining of human testis using 13686-1-AP

IHC staining of human testis using 13686-1-AP

IF Staining of mouse testis using 13686-1-AP

IF Staining of mouse testis using 13686-1-AP

PGK2 Polyclonal antibody

PGK2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.

Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm.

A male germ-cell-specific ribosome controls male fertility

Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate

The Role of Chorein Deficiency in Late Spermatogenesis