Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 17811-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17811-1-AP
WB result of PGK1 antibody (17811-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGK1 transfected HeLa cells.
WB analysis using 17811-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17811-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of SW 1990 using 17811-1-AP
IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:2000) with SW 1990 cells lysate 2800 ug.
IP experiment of HeLa using 17811-1-AP
IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:10000) with HeLa cells lysate 980 ug.
IHC staining of human colon cancer using 17811-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17811-1-AP (PGK1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 17811-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17811-1-AP (PGK1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 17811-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PGK1 antibody (17811-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HepG2 using 17811-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGK1 antibody (17811-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 17811-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug PGK1 Polyclonal antibody (17811-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Featured Product
KD/KO Validated

PGK1 Polyclonal antibody

PGK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 17811-1-AP

Publications(126)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

Cell migration-inducing gene 10 protein, EC:2.7.2.3, OK/SW-cl.110, PGKA, PRP 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (126)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, PC-12 cells, PC-3 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, mouse testis tissue, rat testis tissue
Positive IP detected in	SW 1990 cells, HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 13 publications below
WB	See 105 publications below
IHC	See 27 publications below
IF	See 12 publications below
IP	See 9 publications below
ELISA	See 1 publications below
CoIP	See 7 publications below

Product Information

17811-1-AP targets PGK1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12119 Product name: Recombinant human PGK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-417 aa of BC103752 Sequence: MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI 相同性解析による交差性が予測される生物種
Full Name	phosphoglycerate kinase 1
Calculated molecular weight	417 aa, 45 kDa
Observed molecular weight	40-45 kDa
GenBank accession number	BC103752
Gene Symbol	PGK1
Gene ID (NCBI)	5230
RRID	AB_2161218
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00558
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.

Protocols

Product Specific Protocols
FC protocol for PGK1 antibody 17811-1-AP	Download protocol
IF protocol for PGK1 antibody 17811-1-AP	Download protocol
IHC protocol for PGK1 antibody 17811-1-AP	Download protocol
IP protocol for PGK1 antibody 17811-1-AP	Download protocol
WB protocol for PGK1 antibody 17811-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li View Article
human	WB,IHC	Mol Cancer Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy. Authors - Qiang Cai KD Validated View Article
mouse,human	WB	Cell Metab The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity Authors - Xuedan Sun View Article
human	WB	Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Authors - Linchong Sun View Article
mouse	WB	Bioact Mater Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes. Authors - Yazhen Li View Article
human	WB	Nat Cell Biol An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus Authors - Albertas Navickas View Article

Validation Data Gallery

WB analysis using 17811-1-AP

WB analysis of HeLa using 17811-1-AP

WB analysis using 17811-1-AP

WB analysis of HepG2 using 17811-1-AP

IP experiment of SW 1990 using 17811-1-AP

IP experiment of HeLa using 17811-1-AP

IHC staining of human colon cancer using 17811-1-AP

IHC staining of human colon cancer using 17811-1-AP

IF Staining of A431 using 17811-1-AP

IF Staining of HepG2 using 17811-1-AP

FC experiment of HeLa using 17811-1-AP

PGK1 Polyclonal antibody

PGK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.

The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes.

An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus

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