Validation Data Gallery
Tested Applications
| Positive IHC detected in | human placenta tissue, human meningioma tissue, human kidney tissue, human breast cancer tissue, mouse kidney tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| IHC | See 12 publications below |
| IF | See 12 publications below |
Product Information
10642-1-AP targets PGF/PLGF in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0969 Product name: Recombinant human PGF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC007789 Sequence: MPVMRLFPCFLQLLAGLALPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERRRPKGRGKRRREKQRPTDCHLCGDAVPRR 相同性解析による交差性が予測される生物種 |
| Full Name | placental growth factor |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC007789 |
| Gene Symbol | PGF |
| Gene ID (NCBI) | 5228 |
| RRID | AB_2161061 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Placenta growth factor (PLGF) is also named as PGF and PGFL. PGF is a homodimeric glycoprotein, 46-50 kDa in size, belonging to the vascular endothelial growth factor (VEGF) sub-family. It plays an important role in pathological VEGF-driven angiogenesis. PLGF is expressed not only in placental cells but also in colon and mammary carcinomas. It acts as a primary inflammatory instigator of atherosclerotic plaque instability and thus may be useful as a risk-predicting biomarker in patients with acute coronary syndromes (ACS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PGF/PLGF antibody 10642-1-AP | Download protocol |
| IHC protocol for PGF/PLGF antibody 10642-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomaterials Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.
| ||
Brain. Global gene expression profiling of somatic motor neuron populations with different vulnerability identify molecules and pathways of degeneration and protection. | ||
Int J Biol Sci Identification of a novel cancer stem cell subpopulation that promotes progression of human fatal renal cell carcinoma by single-cell RNA-seq analysis. | ||
Int J Biol Macromol Molecular mechanism of Flt-1 protein and the regulation of monocytes modulate endothelial cell in wound healing sites via PGF/FLT1 signaling | ||
Environ Res Prenatal triphenyl phosphate exposure impairs placentation and induces preeclampsia-like symptoms in mice |