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CoraLite® Plus 647-conjugated PGC1a Monoclonal antibody
PGC1a Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1C1B2
Cat no : CL647-66369
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL647-66369 targets PGC1a in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | peroxisome proliferator-activated receptor gamma, coactivator 1 alpha |
Calculated molecular weight | 91 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | NM_013261 |
Gene symbol | PPARGC1A |
Gene ID (NCBI) | 10891 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 PGC1a antibody CL647-66369 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |