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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of human testis using 55392-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of mouse testis using 55392-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of HEK-293 using 55392-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of MCF-7 using 55392-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of mouse testis using 55392-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human testis using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human testis using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human breast cancer using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human breast cancer using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PGAP1 Polyclonal antibody (55392-1-AP)

    IF Staining of HeLa using 55392-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PGAP1 antibody (55392-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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PGAP1 Polyclonal antibody

PGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55392-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Bst1, GPI inositol deacylase, hPGAP1, ISPD3024, PGAP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, HEK-293 cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55392-1-AP targets PGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name post-GPI attachment to proteins 1
Calculated molecular weight 105 kDa
Observed molecular weight 49-53 kDa, 85-90 kDa
GenBank accession numberNM_024989
Gene Symbol PGAP1
Gene ID (NCBI) 80055
RRIDAB_11232412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ75T13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1.

Protocols

Product Specific Protocols
WB protocol for PGAP1 antibody 55392-1-APDownload protocol
IHC protocol for PGAP1 antibody 55392-1-APDownload protocol
IF protocol for PGAP1 antibody 55392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
HumanIHC

Nature

A single-cell atlas of human and mouse white adipose tissue.

Authors - Margo P Emont
View Article
humanWB

Mol Cell

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Authors - Tatsuaki Kurosaki
View Article
humanWB

Cancer Med

Identification of novel pQTL-SNPs associated with lung adenocarcinoma risk: A multi-stage study

Authors - Yutong Wu
View Article
humanWB

J Biol Chem

Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.

Authors - Takahiro Nakane
View Article