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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGAM2 Polyclonal antibody

PGAM2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25518-1-AP

Synonyms

BPG dependent PGAM 2, PGAM M, PGAM2, PGAMM, Phosphoglycerate mutase 2

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 25518-1-AP

    WB analysis of mouse testis using 25518-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25518-1-AP (PGAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

25518-1-AP targets PGAM2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate mutase 2 (muscle)
Calculated molecular weight 28 kDa
Observed molecular weight 29 kDa
GenBank accession numberNM_000290
Gene symbol PGAM2
Gene ID (NCBI) 5224
RRIDAB_2880115
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody is specific for PGAM2.

Protocols

Product Specific Protocols
WB protocol for PGAM2 antibody 25518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols