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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PFTK1 Polyclonal antibody

PFTK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21612-1-AP

Synonyms

CDK14, hPFTAIRE1, KIAA0834, PFTAIRE protein kinase 1, PFTAIRE1, PFTK1

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 21612-1-AP

    WB analysis of HeLa using 21612-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 21612-1-AP

    WB analysis of SH-SY5Y using 21612-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 21612-1-AP

    WB analysis of mouse brain using 21612-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21612-1-AP

    IHC staining of human kidney using 21612-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 21612-1-AP

    IHC staining of human kidney using 21612-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse brain using 21612-1-AP

    IHC staining of mouse brain using 21612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 21612-1-AP

    IHC staining of mouse brain using 21612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 21612-1-AP

    IHC staining of human kidney using 21612-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 21612-1-AP

    IHC staining of human kidney using 21612-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

21612-1-AP targets PFTK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFTK1 fusion protein Ag16259 相同性解析による交差性が予測される生物種
Full Name PFTAIRE protein kinase 1
Calculated molecular weight 469 aa, 53 kDa
Observed molecular weight 48-53 kDa
GenBank accession numberBC136477
Gene symbol PFTK1
Gene ID (NCBI) 5218
RRIDAB_10858924
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFTK1 is a Cdc2-related protein kinase, also named as PFTAIRE1, CDK14 and belongs to the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It acts as a cyclin-dependent kinases that regulates cell cycle progression and cell proliferation. The PFTK1 protein shows high homology with mouse Pftaire1, a Cdc2-related protein expressed primarily in the postnatal and adult nervous system(PMID:9202329). The protein contains 2 predicted nuclear localization signals that the N-terminal nuclear localization signals do not play a role in vivo(PMID:11313143). It has 3 isoforms produced by alternative splicing. This antibody is specific to PFTK1.

Protocols

Product Specific Protocols
WB protocol for PFTK1 antibody 21612-1-APDownload protocol
IHC protocol for PFTK1 antibody 21612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Oncolytics

Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus

Authors - Dakota W. Goad
View Article
mouseWB, IHC, IF

Toxicol Lett

CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.

Authors - Daniel Pollack
View Article
humanWB

World Neurosurg

MiR-205 Inhibits Sporadic Vestibular Schwannoma Cell Proliferation by Targeting Cyclin-Dependent Kinase 14.

Authors - Xiaoling Yin
View Article
humanWB,IHC

Biomed Pharmacother

MiR-542-3p, a microRNA targeting CDK14, suppresses cell proliferation, invasiveness, and tumorigenesis of epithelial ovarian cancer.

Authors - Jingwei Li
View Article