Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25188-1-AP targets PFN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18131 Product name: Recombinant human PFN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-137 aa of BC132952 Sequence: MGDWKVYISAVLRDQRIDDVAIVGHADNSCVWASRPGGLLAAISPQEVGVLTGPDRHTFLQAGLSVGGRRCCVIRDHLLAEGDGVLDARTKGLDARAVCVGRAPRALLVLMGRRGVHGGILNKTVHELIRGLRMQGA 相同性解析による交差性が予測される生物種 |
| Full Name | profilin 3 |
| Calculated molecular weight | 137 aa, 15 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC132952 |
| Gene Symbol | PFN3 |
| Gene ID (NCBI) | 345456 |
| RRID | AB_3742139 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60673 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PFN3 also known as profilin-3, belongs to the profilin family, a family of actin-binding proteins that regulate the dynamics of actin in cells. PFN3 may be involved in spermatogenesis. (PMID: 11867228). Deletion or abnormal function of PFN3 may lead to male infertility, especially in disorders associated with abnormal sperm acrosome development (spherozoospermia)(PMID: 34869336).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PFN3 antibody 25188-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |