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PFKM Polyclonal antibody

PFKM Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55028-1-AP

Synonyms

GSD7, PFK 1, PFK A, PFK M, PFKM, PFKX, Phosphofructokinase 1, Phosphofructokinase M, phosphofructokinase, muscle, Phosphohexokinase



Tested Applications

Positive WB detected inHeLa cells, human skeletal muscle tissue, HepG2 cells, human brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue, mouse liver tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

55028-1-AP targets PFKM in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, chicken, rat, mouse, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, muscle
Calculated molecular weight 85 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberNM_000289
Gene symbol PFKM
Gene ID (NCBI) 5213
RRIDAB_10858390
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.

Protocols

Product Specific Protocols
WB protocol for PFKM antibody 55028-1-APDownload protocol
IHC protocol for PFKM antibody 55028-1-APDownload protocol
IF protocol for PFKM antibody 55028-1-APDownload protocol
IP protocol for PFKM antibody 55028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
humanWB

Nature

Mechanical regulation of glycolysis via cytoskeleton architecture.

Authors - Jin Suk Park
humanWB

Nat Methods

A large-scale targeted proteomics assay resource based on an in vitro human proteome.

Authors - Masaki Matsumoto
mouseIF

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
humanWB

Biomaterials

Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.

Authors - Meiling Wu
humanWB

Cancer Res

Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer.

Authors - Jiao Wang